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Journal: European journal of endocrinology

Article Title: ABIRATERONE ACETATE TREATMENT LOWERS 11-OXYGENATED ANDROGENS

doi: 10.1530/EJE-19-0905

Figure Lengend Snippet: List of steroid analytes, standards, internal standards, and mass spectrometry parameters

Article Snippet: Mobile phases consisted of 0.2 mmol/L aqueous ammonium fluoride (phase A) and methanol with 0.2 mmol/L ammonium fluoride (phase B). table ft1 table-wrap mode="anchored" t5 caption a7 Steroid Source MRM (m/z) Qual (m/z) RT (min) LLOQ (nmol/L) Progesterone Cerilliant 315.2/97.0 109.1 14.4 0.09 Progesterone-d9 C/D/N Isotopes 324.2/97.0 113.1 14.4 17-Hydroxyprogesterone Cerilliant 331.2/97.0 313.0 11.4 0.1 17-Hydroxyprogesterone-d8 C/D/N Isotopes 339.2/100.0 113.0 11.3 Corticosterone Cerilliant 347.2/121.1 329.2 10.6 0.6 Corticosterone-d8 C/D/N Isotopes 355.2/337.1 125.1 10.5 11-Deoxycorticosterone Cerilliant 331.2/97.0 109.0 13.0 0.06 11-Deoxycorticosterone-d8 Sigma-Aldrich 339.2/100.0 113.0 13.0 11-Deoxycortisol Cerilliant 347.2/97.0 109.0 10.0 0.01 11-Deoxycortisol-d5 Sigma-Aldrich 352.2/100.1 113.1 9.9 Cortisol Cerilliant 363.2/121.1 327.3 7.3 0.6 Cortisol-d4 Cerilliant 367.2/121.1 97.0 7.3 Cortisone Cerilliant 361.2/163.0 121.1 8.0 0.7 Cortisone-d8 Cerilliant 369.0/169.0 120.0 7.9 Androstenedione Cerilliant 287.0/97.0 109.0 13.0 0.03 Androstenedione-d7 Cambridge Isotopes 294.0/100.0 113.0 13.0 Testosterone Cerilliant 289.2/97.0 109.0 11.3 0.02 Testosterone-d3 C/D/N Isotopes 292.0/97.0 109.0 11.3 11-Hydroxyandrostenedione Steraloids 303.2/120.9 285.1 10.5 0.3 11-Hydroxyandrostenedione-d3 RTI 306.2/288.2 122.1 10.4 11-Ketoandrostenedione Steraloids 301.2/257.0 120.9 or 265.1 10.7 0.1 11-Hydroxyandrostenedione-d3 RTI 306.2/288.2 122.1 10.4 11-Hydroxytestosterone Steraloids 305.3/269.0 105.0 10.5 0.1 11-Ketotestosterone-d3 RTI 308.3/262.3 123.1 9.3 11-Ketotestosterone Steraloids 303.2/120.9 259.1 9.3 0.1 11-Ketotestosterone -d3 RTI 308.3/262.3 123.1 9.3 Open in a separate window MRM, precursor and product ions for mass ratio monitoring; Qual, qualifier ion; m/z, mass to charge ratio; RT retention time; LLOQ, lower limit of quantitation (extrapolated signal-to-noise ratio of 3); RTI, The National Heart.

Techniques: Mass Spectrometry